In vivo Reconstitution of Algal Triacylglycerol Production in Saccharomyces cerevisiae

نویسندگان

  • Chun-Hsien Hung
  • Kazue Kanehara
  • Yuki Nakamura
چکیده

The current fascination with algal biofuel production stems from a high lipid biosynthetic capacity and little conflict with land plant cultivation. However, the mechanisms which enable algae to accumulate massive oil remain elusive. An enzyme for triacylglycerol (TAG) biosynthesis in Chlamydomonas reinhardtii, CrDGTT2, can produce a large amount of TAG when expressed in yeast or higher plants, suggesting a unique ability of CrDGTT2 to enhance oil production in a heterologous system. Here, we performed metabolic engineering in Saccharomyces cerevisiae by taking advantage of CrDGTT2. We suppressed membrane phospholipid biosynthesis at the log phase by mutating OPI3, enhanced TAG biosynthetic pathway at the stationary phase by overexpressing PAH1 and CrDGTT2, and suppressed TAG hydrolysis on growth resumption from the stationary phase by knocking out DGK1. The resulting engineered yeast cells accumulated about 70-fold of TAG compared with wild type cells. Moreover, TAG production was sustainable. Our results demonstrated the enhanced and sustainable TAG production in the yeast synthetic platform.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Statistical Optimization of Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology

In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of ...

متن کامل

Kinetics Studies Impact of Initial pH and Addition of Yeast Saccharomyces cerevisiae on Biogas Production from Tofu Wastewater in Indonesia

The purpose of this work was to study the effect of initial pH and yeast Saccharomyces Cerevisiae on biogas production from tofu wastewater (TW). The initial pH was varied in ranging of 5 – 9 in substrate without yeast (T5-T9) and with yeast (TY5-TY9). The results showed that optimum initial pH was 8. The maximum biogas was resulted in T8 (275 mL) and TY8 (421 mL). Yeast addition increased tota...

متن کامل

Statistical Optimization of Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology

In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of ...

متن کامل

Production of Single Cell Protein from Sugarcane Bagasse by Saccharomyces cerevisiae in Tray Bioreactor

In this study, solid state fermentation (SSF) was carried out to produce single cell protein (SCP) from sugarcane bagasse using Saccharomyces cerevisiae. The SSF experiment were performed in a tray bioreactor. The influence of several parameters including extraction buffer, initial moisture content of substrate, fermentation time, relative humidity in bioreactor, the bioreactor temperature and ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2016